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biotinylated anti kim 1 detection antibody  (R&D Systems)


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    R&D Systems biotinylated anti kim 1 detection antibody
    Biotinylated Anti Kim 1 Detection Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+kim+1+detection+antibody/Mouse+TIM-1%2FKIM-1%2FHAVCR+Biotinylated+Antibody/us10712349-1217-14-26
    Average 91 stars, based on 8 article reviews
    biotinylated anti kim 1 detection antibody - by Bioz Stars, 2026-09
    91/100 stars

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    Incubation:

    Article Title: Circulating KIM-1 levels for detection of pathologies associated with injury to, or cancer of, the kidney
    Article Snippet: Approximately 6000 beads in 50 ml of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 ml of sample or recombinant KIM-1 protein (1817-TM-050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women's Hospital]) for 45 minutes. .. After incubation, beads were washed three times with PBS with Tween and incubated with biotinylated anti-KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women's Hospital]) for 30 minutes. .. Beads were washed again with PBS with Tween and incubated for 15 minutes with the streptavidin-phycoerythrin solution (Invitrogen).

    Article Title: Novel Assays for Detection of Urinary KIM-1 in Mouse Models of Kidney Injury
    Article Snippet: Approximately 6000 beads in 50 μl of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% bovine serum albumin [BSA]; pH 7.4; filter sterilized) were incubated with 30 μl of sample or recombinant mouse KIM-1 protein (rmKIM-1, R&D systems, catalog no. 1817-TM-050-CF) for 45min. .. After incubation, beads were washed thrice with PBST Tween-PBS and incubated with biotinylated anti-KIM-1 detection antibody (R&D systems, catalog no. BAF 1817) for 30 min. Beads were washed again with PBST and incubated for 15min with the streptavidin-PE solution (Invitrogen). .. The signal from the fluorochrome, which is directly proportional to the amount of antigen bound at the microbead surface, is captured using the Bio-Plex system (Bio-Rad).



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